What are the Key Components of Fluorescent Multiplexing?
The key components of fluorescent multiplexing include:
Fluorophores: These are the fluorescent molecules that emit light upon excitation. Commonly used fluorophores include FITC, TRITC, and Alexa Fluor dyes. Antibodies: These are used to specifically bind to the target molecules of interest. They are often conjugated to fluorophores for detection. Microscopy: A fluorescence microscope equipped with the appropriate filters and detectors is essential for capturing the emitted light from the fluorophores. Software: Advanced imaging software is used to analyze and interpret the multiplexed data, allowing for the separation and quantification of signals from different fluorophores.
