The process of conducting a reporter gene assay involves several steps:
Construct Design: A DNA construct is designed, linking the reporter gene to the promoter or regulatory sequence of interest. Transfection: The construct is introduced into the cells or tissues, often using techniques like viral transduction or electroporation. Expression Monitoring: The expression of the reporter gene is monitored using appropriate detection methods, such as fluorescence microscopy or luminescence assays. Data Analysis: Quantitative and qualitative data are analyzed to interpret gene expression patterns and regulatory mechanisms.
