To maintain sample quality, several factors must be considered:
- Temperature Control: Consistent low temperatures are crucial for preventing degradation. For cryopreservation, samples should be stored at -80°C or lower. - Humidity Control: Excess moisture can cause mold and other issues. Desiccants or controlled environments can help mitigate this. - Proper Labeling: Clear labeling with relevant information such as date, type of tissue, and fixation method is essential for future identification and use. - Avoiding Repeated Freeze-Thaw Cycles: These cycles can damage tissues and degrade biomolecules. Samples should be aliquoted whenever possible to avoid this issue.