1. Antibody Specificity: Ensuring that the antibodies used in IHC and other techniques are specific to L1CAM and do not cross-react with other proteins is crucial. 2. Tissue Processing: Proper fixation and processing of tissues are essential to preserve L1CAM epitopes for accurate detection. 3. Interpretation of Results: Understanding the physiological versus pathological expression of L1CAM requires careful analysis and comparison with controls.
