What are the Challenges in Secondary Cell Culture?
Secondary cell culture, while beneficial, comes with its own set of challenges:
- Contamination: There is a risk of bacterial, fungal, or mycoplasma contamination, which can compromise the integrity of the culture. - Cell Senescence: Over time, cells may undergo senescence, losing their ability to proliferate. - Genetic Drift: Prolonged culturing can lead to genetic alterations, potentially affecting the behavior and characteristics of the cells.
