The process of secondary cell culture involves several steps, including:
1. Observation: Monitoring the primary culture to determine the optimal time for subculturing, usually when cells reach a certain confluence (around 70-80%). 2. Detachment: Using enzymes like trypsin or EDTA to detach adherent cells from the culture vessel. 3. Resuspension: Resuspending the detached cells in fresh culture medium. 4. Seeding: Transferring the cell suspension to a new culture vessel at a lower density to allow for further growth.
