Reverse transcription is a powerful technique, but it comes with challenges:
- RNA Degradation: RNA is highly susceptible to degradation by RNases, which can lead to loss of sample integrity. - Primer Design: Designing specific primers that efficiently bind to the RNA template is crucial for successful cDNA synthesis. - Efficiency of Reverse Transcriptase: The efficiency of the reverse transcriptase enzyme can vary, affecting the yield and quality of cDNA. - Contamination: Contamination with genomic DNA or other sources can lead to inaccurate results.
