What are the Key Steps in the Reverse Transcription Process?
The reverse transcription process involves the following key steps:
1. RNA Template Preparation: RNA extracted from tissue samples is prepared and quantified. 2. Primer Annealing: Short DNA primers complementary to the RNA template are added to initiate the synthesis of cDNA. 3. Reverse Transcriptase Addition: The enzyme reverse transcriptase is added to catalyze the synthesis of cDNA from the RNA template. 4. cDNA Synthesis: The reverse transcriptase enzyme synthesizes cDNA by extending the primers. 5. Termination: The reaction is terminated, and the newly synthesized cDNA is ready for further analysis.
