Despite their importance, proliferation studies come with several challenges:
1. Specificity and Sensitivity: Choosing the right markers and techniques is crucial for obtaining accurate results. Some markers may not be specific to proliferation alone and could lead to false positives.
2. Technical Variability: Differences in tissue processing, staining protocols, and antibody quality can affect the consistency of results.
3. Interpretation: Proper interpretation of proliferation data requires expertise, as various factors can influence cell division, including the microenvironment and cellular context.
