Detecting cell line drift involves regular monitoring of cell line characteristics through several approaches:
Genetic Profiling: Techniques such as Short Tandem Repeat (STR) profiling are used to confirm the genetic identity of cell lines. Phenotypic Analysis: Observing changes in morphology, growth rates, and cell markers can help identify drift. Transcriptomic and Proteomic Studies: Analyzing RNA and protein expression patterns can reveal underlying changes.