What are the applications of Nitroblue Tetrazolium in Histology?
NBT has several important applications in histology, including:
1. Detection of Enzyme Activity: NBT is commonly used to detect the activity of certain enzymes, such as those in the mitochondrial respiratory chain. 2. Assessment of Cellular Oxidative Stress: It is employed to measure the oxidative burst in phagocytes, which is crucial for understanding immune responses. 3. Histochemical Staining: NBT is used in histochemical staining procedures to visualize specific cell types and their metabolic activities.
