Are there any limitations to using Nitroblue Tetrazolium?
While NBT is a powerful tool, it does have some limitations:
1. Potential for Non-Specific Staining: In some cases, NBT can produce non-specific staining, which can complicate the interpretation of results. 2. Sensitivity to Light: The blue formazan product is light-sensitive and can degrade over time, necessitating prompt analysis to avoid inaccuracies. 3. Toxicity: NBT and its reduced form can be toxic to cells, which may affect cell viability and experimental outcomes.
