Although BrdU is widely used, several alternatives exist: 1. EdU (5-ethynyl-2'-deoxyuridine): A thymidine analog similar to BrdU, but with a simpler detection method that does not require DNA denaturation, making it less damaging to cells. 2. Ki-67: A protein expressed in proliferating cells, Ki-67 can be detected using specific antibodies and serves as a marker for cell proliferation. 3. PCNA (Proliferating Cell Nuclear Antigen): Another marker for cell proliferation, PCNA is involved in DNA replication and repair and can be detected through immunostaining techniques.
