Detection of BrdU involves several steps. After the incorporation period, DNA is typically denatured to make the incorporated BrdU accessible to antibodies. This can be done using acid, heat, or enzymatic treatment. The samples are then incubated with a primary antibody specific for BrdU. A secondary antibody conjugated to a fluorescent dye or an enzyme is used to visualize the BrdU incorporation, allowing for quantitative and qualitative analysis using fluorescence microscopy or other imaging techniques.
