Specificity: Using highly specific antibodies allows for precise localization of target antigens. Versatility: IHC can be applied to a wide range of tissues and conditions. Quantitative Analysis: Image analysis software can quantify staining intensity and distribution.
Limitations:
Antibody Quality: The success of IHC depends heavily on the quality and specificity of the antibodies used. Technical Variability: Differences in tissue processing, antigen retrieval, and staining protocols can affect reproducibility. Background Staining: Non-specific binding can sometimes lead to high background staining, complicating interpretation.
