What Are the Controls Used in Immunohistochemistry?
Proper controls are essential to validate the specificity and accuracy of IHC results:
Positive Control: A tissue known to express the target antigen ensures that the staining protocol works effectively. Negative Control: A tissue known not to express the target antigen confirms the specificity of the primary antibody. Isotype Control: An antibody of the same isotype as the primary antibody but irrelevant to the antigen helps identify non-specific binding.
