What Are the Advantages and Limitations of Confocal Microscopy?
Confocal microscopy offers several advantages, including improved resolution and contrast, the ability to produce three-dimensional reconstructions, and reduced background noise by excluding out-of-focus light. However, it also has some limitations, such as:
Limited penetration depth, making it less effective for imaging thick samples. Potential phototoxicity and photobleaching due to prolonged exposure to intense light. Higher cost and complexity compared to traditional light microscopy.
