How is Multiphoton Microscopy Different from Conventional Fluorescence Microscopy?
Multiphoton microscopy differs from conventional fluorescence microscopy by using two or more photons of longer wavelengths (usually infrared) to excite fluorophores. This method reduces photodamage and allows imaging of living tissues at greater depths, making it suitable for intravital imaging. The longer wavelengths also cause less scattering, resulting in clearer images of thick specimens.
