What Are Some Common Fluorophores Used in Histology?
Several fluorophores are commonly used in histology, each with unique properties:
- Fluorescein (FITC): Emits green fluorescence and is often used in immunofluorescence. - Texas Red: Emits red fluorescence and is used for labeling proteins and nucleic acids. - DAPI: Binds to DNA and emits blue fluorescence, commonly used for nuclear staining. - mCherry: A red fluorescent protein used in live-cell imaging and reporter assays.
