What Are Some Techniques to Overcome Photobleaching?
Several strategies can be employed to minimize photobleaching:
- Use of Antifade Reagents: These chemicals can be added to the mounting medium to reduce photobleaching. - Minimize Light Exposure: Reducing the exposure time and light intensity can help preserve fluorescence. - Use of More Stable Fluorophores: Some newer fluorophores are designed to be more resistant to photobleaching.