Pimonidazole adducts refer to the covalent modifications that occur when the hypoxia marker pimonidazole binds to cellular macromolecules under low oxygen conditions. Pimonidazole is a 2-nitroimidazole compound that is reduced and forms stable adducts with thiol-containing proteins, peptides, and amino acids in hypoxic cells. These adducts can be detected using immunohistochemical techniques, making them valuable tools in histological studies of tissue hypoxia.
