How are Pimonidazole Adducts Detected in Histology?
The detection of pimonidazole adducts in histological samples typically involves immunohistochemistry (IHC). After administering pimonidazole to the subject (either in vivo or in vitro), tissue samples are collected and processed. Sections of the tissue are then incubated with specific antibodies that recognize pimonidazole adducts. These antibodies are usually conjugated to a detectable marker, such as an enzyme or a fluorescent dye. The presence of hypoxia can then be visualized under a microscope by the localization of the staining.
