Multiplex staining techniques involve the use of multiple stains or antibodies to detect different antigens or markers within the same tissue section. This allows for the simultaneous visualization of various cellular components and interactions. Techniques like immunohistochemistry (IHC) can be combined with fluorescence in situ hybridization (FISH) to study both protein expression and genetic alterations in the same sample. Multiplex staining provides a more comprehensive understanding of the tissue's molecular landscape and is particularly useful in cancer research and diagnostics.
