Image analysis software works by processing and analyzing digital images of histological slides to extract meaningful data. These programs use algorithms to identify and quantify specific features within the tissue, such as cell counts, area measurements, and staining intensities. The software can be customized to recognize different types of cells and structures, making it highly versatile for various applications. For example, CellProfiler can be used to analyze cell morphology and quantify fluorescence signals, while Halo offers tools for analyzing tissue microarrays and whole-slide images.