What are common pitfalls in histological analysis?
Histological analysis can be challenging, and several pitfalls should be avoided:
- Improper Fixation: Poor fixation can lead to tissue degradation and artifacts that obscure important details. - Over-staining or Under-staining: Incorrect staining can make it difficult to distinguish between different tissue components. - Sectioning Artifacts: Poor sectioning techniques can cause tissue distortion, folds, or tears that complicate analysis. - Non-specific Binding in IHC: Non-specific antibody binding can produce misleading results, so proper controls are essential.
