How do you prepare tissue samples for histological examination?
Preparing tissue samples involves several steps:
1. Fixation: Preserves the tissue's structure and prevents degradation using chemicals like formaldehyde. 2. Embedding: The tissue is embedded in a solid medium like paraffin wax to provide support during slicing. 3. Sectioning: Thin slices of the embedded tissue are cut using a microtome. 4. Staining: Tissues are stained with dyes such as hematoxylin and eosin to enhance contrast and visualize different components under a microscope.
