Using a block holder involves several steps to ensure proper sectioning:
Prepare the Tissue Block: Make sure the paraffin-embedded tissue is properly trimmed and fits well within the block holder. Adjust the Holder: Secure the tissue block in the holder and adjust any settings to ensure it is tightly held. Mount on Microtome: Place the block holder onto the microtome, ensuring it is properly aligned for sectioning. Section the Tissue: Proceed with cutting thin sections of the tissue using the microtome's blade.
