Common staining issues and their solutions include:
Over-staining: This can be resolved by shortening staining times or using a differentiating agent more effectively. li> Under-staining: Increase the staining time or the concentration of the staining solution. Non-specific staining: This can be minimized by using blocking agents in immunohistochemistry or optimizing washing steps in other staining protocols. Artifacts: Ensure proper tissue handling and preparation. Artifacts can also be reduced by optimizing dehydration and clearing steps.
