How to Optimize Hematoxylin and Eosin (H&E) Staining?
To optimize H&E staining:
Fixation: Ensure proper fixation of tissue samples to preserve cellular structures. Formalin is commonly used. Sectioning: Cut tissue sections at an appropriate thickness, typically 4-5 micrometers, to ensure adequate staining penetration. Staining Time: Adjust staining times for hematoxylin and eosin to get optimal contrast. Over-staining or under-staining can obscure details. Differentiation: Use a differentiating agent to remove excess stain, ensuring clear nuclear and cytoplasmic distinction. Dehydration: Properly dehydrate and clear sections before mounting to avoid artifacts.
