What Challenges are Faced in Protein Purification?
Protein purification can be challenging due to factors such as:
- Protein Stability: Proteins can denature or degrade during the purification process. - Complex Mixtures: Tissue samples contain a diverse array of proteins, making it difficult to isolate the target protein. - Yield and Purity: Achieving high yield and purity often requires optimization of the purification protocol. - Contaminants: Removing contaminants that co-purify with the target protein can be challenging.
