What Techniques Are Used in Histology to Study Cognitive Dysfunction?
Light Microscopy Light microscopy enables the visualization of tissue architecture and the identification of major cellular components. Staining techniques, such as hematoxylin and eosin (H&E) staining, help differentiate cell types and structures.
Immunohistochemistry (IHC) IHC uses antibodies to detect specific proteins within tissue sections. This technique is particularly useful for identifying abnormal protein accumulations, such as amyloid-beta and tau, that are associated with cognitive dysfunction.
Electron Microscopy Electron microscopy provides high-resolution images of cellular and subcellular structures. It is essential for studying the ultrastructural changes, such as synaptic alterations, that contribute to cognitive impairments.
Fluorescence Microscopy Fluorescence microscopy allows for the visualization of specific proteins or cellular components tagged with fluorescent markers. This technique is valuable for studying dynamic processes and interactions within neurons and glial cells.
