Preventing chatter artifacts involves addressing the factors that cause them:
Blade Maintenance: Ensure the microtome blade is sharp and free of nicks or other damage. Regularly changing the blade can help maintain cutting quality. Optimal Cutting Speed: Adjust the cutting speed to a moderate pace to minimize vibrations and ensure smooth sectioning. Proper Tissue Processing: Ensure that tissues are adequately fixed, processed, and embedded to achieve optimal hardness for sectioning. Correct Block Orientation: Properly orient the tissue block to minimize resistance and achieve smooth cutting.
