ribonucleases

How to Inhibit Ribonucleases in Histological Procedures?


To prevent RNA degradation during histological procedures, it is essential to inhibit RNase activity. Several strategies can be employed:
Use of RNase inhibitors: Chemical inhibitors such as diethyl pyrocarbonate (DEPC) can be used to inactivate RNases.
Proper sample handling: Use RNase-free reagents and consumables, and work in an RNase-free environment to minimize contamination.
Tissue fixation: Fixatives such as formaldehyde can help preserve RNA by cross-linking proteins and nucleic acids, thereby inhibiting RNase activity.
Storage conditions: Store samples at low temperatures (e.g., -80°C) to reduce RNase activity.

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