How do Ribonucleases Affect Histological Techniques?
Ribonucleases can significantly impact various histological techniques if not properly controlled. For instance, during RNA extraction and purification, RNases can degrade RNA, leading to poor yield and quality of RNA samples. This can affect downstream applications such as qPCR, microarray analysis, and next-generation sequencing.
In tissue sectioning and staining, the presence of RNases can result in the degradation of RNA, which is particularly problematic for techniques that rely on the detection of RNA, such as in situ hybridization or RNAscope.
