When documenting histological techniques, specificity is crucial. Here are some tips:
- Sample Preparation: Describe the source and type of the tissue, fixation method (e.g., formalin fixation), and tissue processing steps. - Embedding and Sectioning: Note the embedding medium (e.g., paraffin), section thickness, and microtome settings. - Staining Protocols: Provide detailed staining procedures, including the type of stain (e.g., Hematoxylin and Eosin), concentrations, incubation times, and any modifications. - Microscopy: Record the type of microscope used, magnification levels, and imaging techniques (e.g., brightfield, fluorescence).