Choosing the right organic solvent depends on several factors, including the type of tissue, the staining protocol, and the desired outcome. Here are some considerations:
Compatibility: Ensure that the solvent is compatible with the tissue type and the staining reagents. Safety: Consider the toxicity and flammability of the solvent. For example, xylene is effective but highly toxic, while toluene is a safer alternative. Effectiveness: The solvent should effectively dehydrate and clear the tissue without causing shrinkage or distortion.
