What are the Challenges in Using Fluorescence Imaging Software?
Despite its advantages, using fluorescence imaging software in histology comes with certain challenges:
Data Complexity: Fluorescence microscopy generates large, complex datasets that require significant computational resources for processing and analysis. Standardization: Variations in sample preparation, imaging conditions, and software settings can lead to inconsistent results. Standardization protocols are essential for reproducibility. User Expertise: Effective use of the software requires a certain level of expertise in both histology and computational analysis. Cost: High-quality commercial software can be expensive, which may be a barrier for some laboratories.
