Before slicing, tissues must undergo several preparatory steps:
1. Fixation: This process involves treating tissues with chemicals like formaldehyde to preserve their structure. 2. Embedding: Tissues are embedded in a supportive medium, such as paraffin wax or resin, to provide stability during slicing. 3. Sectioning: The embedded tissue is placed in a microtome, cryostat, or vibratome, and thin sections are cut.
