Several factors can affect the quality of tissue slices:
- Thickness: The thickness of the slices can influence the quality of microscopic images. Typically, sections are cut between 1-10 micrometers. - Sharpness of the Blade: A sharp blade is essential for producing clean, smooth sections. - Temperature: For cryostat sectioning, maintaining the correct temperature is crucial to prevent tissue distortion. - Embedding Medium: The choice of embedding medium can impact the ease of sectioning and the quality of the slices.
