How is tissue preparation carried out for histological analysis?
Tissue preparation involves several steps to ensure the preservation and accurate representation of tissue morphology:
- Fixation: Preserves tissue structure by cross-linking proteins, typically using formaldehyde or glutaraldehyde. - Embedding: Tissues are embedded in paraffin or resin to provide support for sectioning. - Sectioning: Thin slices of tissue, typically 5-10 micrometers thick, are cut using a microtome. - Mounting: Sections are placed on glass slides for staining and microscopic examination.
