1. Fixation: Tissues are preserved using chemicals like formaldehyde to prevent degradation. 2. Embedding: Fixed tissues are embedded in a medium such as paraffin wax to provide support during sectioning. 3. Sectioning: Thin slices of the embedded tissue are cut using a microtome. 4. Deparaffinization and Rehydration: Sections are deparaffinized and rehydrated to make them receptive to staining. 5. Staining: Sections are stained using the chosen staining protocol. 6. Dehydration and Clearing: Stained sections are dehydrated and cleared to prepare them for mounting. 7. Mounting: Sections are mounted on slides using a mounting medium and covered with a coverslip.
