The functional layer is studied using various histological techniques, including:
- Histological Staining: Techniques such as Hematoxylin and Eosin (H&E) staining are used to visualize the different cellular components. - Immunohistochemistry: This method employs antibodies to detect specific proteins expressed in the functional layer, providing insights into hormone receptor status and other molecular characteristics. - Electron Microscopy: Provides detailed images of the ultrastructure of cells and tissues within the functional layer.