Histological techniques to study the Akt pathway include immunohistochemistry (IHC) and immunofluorescence, which allow for the visualization and localization of Akt and its phosphorylated forms in tissue sections. Western blotting and ELISA are used to quantify Akt activity and phosphorylation status. Additionally, genetic and pharmacological approaches, such as gene knockouts and specific inhibitors, help elucidate the pathway's role in different tissues.
