Various techniques are used to study spatial histology, including:
Histochemical Staining: Techniques such as Hematoxylin and Eosin (H&E) staining provide information on the general structure and organization of tissues. Immunohistochemistry: This technique uses antibodies to detect specific proteins within tissues, allowing for the visualization of spatial distribution of molecules. In Situ Hybridization: This method detects specific nucleic acid sequences within tissues, providing spatial information on gene expression. Advanced Imaging: Techniques like confocal microscopy and spatial transcriptomics offer high-resolution images and molecular profiling of tissues in their spatial context.
