NSE is commonly detected in histological samples using immunohistochemistry (IHC). This technique involves the use of specific antibodies that bind to NSE, allowing for its visualization under a microscope. The process typically includes the following steps: 1. Tissue Fixation and Sectioning: The tissue sample is fixed, usually with formalin, and then embedded in paraffin. Thin sections are cut from the paraffin block. 2. Antigen Retrieval: The sections are treated to retrieve antigens, making them more accessible for antibody binding. 3. Primary Antibody Application: A primary antibody specific to NSE is applied to the tissue sections. 4. Secondary Antibody Application: A secondary antibody, usually conjugated to a detection molecule such as an enzyme or fluorophore, is applied. 5. Visualization: The enzyme or fluorophore is activated to produce a colorimetric or fluorescent signal, indicating the presence of NSE.
