How is Nanoparticle Toxicity Assessed in Histology?
Several histological techniques are used to assess nanoparticle toxicity:
1. Tissue Staining: Techniques like Hematoxylin and Eosin (H&E) staining are commonly used to examine tissue morphology and identify signs of tissue damage or inflammation.
2. Immunohistochemistry: This technique involves using antibodies to detect specific biomarkers of cellular stress, apoptosis, or inflammation in tissue samples.
3. Electron Microscopy: Transmission and scanning electron microscopy can provide detailed images of nanoparticles within tissues, helping to understand their localization and potential effects at the ultrastructural level.
