In histology, metabolic activity can be measured using various techniques. Some common methods include:
Histochemical staining: Specific stains like PAS (Periodic Acid-Schiff) or Sudan Black can highlight metabolic products such as glycogen and lipids. Immunohistochemistry (IHC): This technique uses antibodies to detect specific enzymes or metabolic proteins, providing a visual representation of metabolic activity. Autoradiography: Involves using radioactive isotopes to trace metabolic pathways and localize metabolic activity within tissues. Fluorescence microscopy: Fluorescent probes can be used to visualize specific metabolic processes in live cells.
