The preparation of histological specimens involves several key steps:
Fixation: This step preserves the tissue by using chemicals like formaldehyde to prevent decay. Embedding: The fixed tissue is embedded in a solid medium like paraffin wax to provide support for thin sectioning. Sectioning: Thin slices of the embedded tissue are cut using a microtome, providing sections that are typically 3-5 micrometers thick. Staining: Stains such as hematoxylin and eosin (H&E) are applied to enhance the contrast of cellular components, making them visible under a microscope.
