Lipidomics involves the extraction, identification, and quantification of lipids from tissue samples. The process typically includes:
1. Sample Preparation: Tissue samples are collected and prepared by homogenization and extraction using solvents like chloroform and methanol. 2. Lipid Extraction: The Bligh and Dyer method or Folch method is commonly used for lipid extraction. 3. Lipid Analysis: Techniques such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy are employed to analyze lipid species. High-performance liquid chromatography (HPLC) is also used to separate lipids before MS analysis.
