Ki-67 labeling is typically performed using immunohistochemistry (IHC). Here are the general steps involved:
1. Tissue samples are fixed and embedded in paraffin. 2. Sections are cut and mounted on slides. 3. Sections are deparaffinized and rehydrated. 4. Antigen retrieval is performed to unmask Ki-67 epitopes. 5. Slides are incubated with a primary antibody against Ki-67. 6. A secondary antibody, conjugated to a detectable marker (like an enzyme or fluorescent dye), is applied. 7. The signal is visualized using chromogenic substrates or fluorescence microscopy.
